602 papers
This study reveals that polyamines act as endogenous buffers for redox-active iron to prevent ferroptosis, establishing a critical molecular link between polyamine metabolism and iron homeostasis.
This study introduces a synthetic spindle assembly checkpoint (SynSAC) synchronization strategy to compare yeast metaphase I and II, revealing that meiosis II features a stronger SAC response and distinct kinetochore composition and phosphorylation patterns compared to meiosis I.
This study reveals that peripheral inflammation triggers the nuclear translocation of the transcription factor Tfe3 in midbrain microglia to upregulate Rab32 expression, which subsequently acts as a physiological rheostat to activate Lrrk2 kinase activity, thereby linking systemic inflammation to Parkinson's disease pathology.
This study demonstrates that supplementing human pluripotent stem cell cultures with a low dosage of the GSK3 inhibitor CHIR99021 prevents long-term aneuploidy and ultra-fine chromosome bridges by fine-tuning WNT signaling, thereby preserving genomic stability and pluripotency for over 30 passages.
This study establishes Protease-Activated Receptor 1 (PAR1) as an endogenous model of functional selectivity, demonstrating that thrombin and activated protein C cleavage differentially engage Gq and G12 signaling pathways to drive distinct physiological outcomes, such as platelet activation versus cytoprotection, without recruiting β-arrestin.
Breast milk extracellular vesicles from mothers with asthma exhibit distinct biophysical properties and differentially modulate inflammatory cytokine release in primary human airway smooth muscle cells depending on the specific asthma status of both the maternal donor and the recipient cell.
This study demonstrates that galectin-1 modulates glomerular endothelial cell adhesion, caveolae formation, and vascular permeability through specific proteomic and phosphoproteomic changes, identifying it as a critical therapeutic target for antibody-mediated kidney rejection.
This study reveals that VAMP8 regulates clathrin-mediated endocytosis not by being recruited into nascent vesicles, but by maintaining endocytic cargo recycling, thereby demonstrating a critical functional link between endocytic recycling and the initiation of endocytosis.
This study identifies "autolamellasomes," a novel receptor-independent autophagy pathway that compacts fragmented endoplasmic reticulum into concentric stacks, thereby explaining the biogenesis of intralysosomal membrane whorls and linking chronic mTOR inhibition to cellular aging and progeria.
This study reveals that Derlin-mediated ERAD prevents the accumulation of the lipid regulator ORMDL3 at ER-mitochondria contact sites, thereby safeguarding mitochondrial function and linking ERAD-dependent spatial control to the pathogenesis of numerous human diseases.
The authors present DF5T, an unsupervised foundation model trained on over 2.25 million electron microscopy images that outperforms existing state-of-the-art methods in five key image enhancement tasks, thereby significantly improving the accuracy of downstream organelle segmentation and 3D structural restoration for biological research.
This study demonstrates that epidermal stem cells utilize the Notch ligand Jag2 to coordinate early differentiation and maintain tissue architecture by signaling to neighboring cells, a process whose disruption leads to compensatory mechanisms that ultimately compromise tissue organization despite preserving barrier function.
This study demonstrates that while both pirfenidone and nintedanib enhance the growth of human alveolar type 2 (AT2) cell organoids, nintedanib uniquely preserves their AT2-like differentiation state and surfactant protein C expression, suggesting it acts proximal to TGF-beta signaling.
This study reveals that medulloblastoma-associated mutations in the E3 ligase adaptor KBTBD4 cause a dual pathological effect by gaining the ability to degrade transcriptional repressors while simultaneously losing the critical function of degrading free PP2A-A scaffolding subunits, thereby disrupting phosphatase quality control and dysregulating key cancer signaling pathways.
Using multi-scale imaging, this study demonstrates that insulin secretory granules dynamically associate with the mitochondrial network over time, a proximity that correlates with and likely drives their maturation through reduced pH, increased biomolecular density, and decreased vesicle diameter.
Using ovarian adenocarcinoma spheroids and computational modeling, this study reveals that collective tissue invasion is driven not by cells pushing through barriers via jamming, but by leader cells inducing apical constriction in the barrier tissue through intercellular integrin adhesions, which mechanically ruptures the barrier.
This study demonstrates that intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors into Pdx1-deficient mouse embryos successfully generates functional interspecies chimeras that produce insulin and extend the mice's lifespan, offering a promising model for regenerative medicine while highlighting the need for improved engraftment efficiency.
This study utilizes a dual genetic switch combining a chimeric G-CSFR:gp130 receptor and hormone-dependent STAT3-ERT2 to identify novel candidate regulators, including IFI16 and IFITM proteins, that orchestrate the transition of human pluripotent stem cells to a naive state through JAK kinase-mediated signaling and epigenomic remodeling.
This study reveals that Homer scaffolding proteins function as polarity-sensitive, phase-separating condensates that integrate Crumbs complex cues to differentially regulate YAP and Wnt signaling pathways through distinct interactions with FRYL and PATJ, thereby coordinating cell growth and transcriptional output in both development and cancer.
This study reveals a novel mechanotransduction paradigm where mechanical stress triggers caveolae disassembly, releasing caveolin-1 scaffolds that remotely inhibit signaling kinases like JAK1 through direct interaction, thereby dynamically regulating cellular responses to tension.